NAD+: which line to draw to

Enter your NAD+ vial amount, the water you added and your dose. NAD+ is dosed in mg; the syringe shows exactly where to stop. New to this?

Your line shows up here.

Fill in the vial, water and dose boxes.

Limited human evidence. Sold as a supplement; injected use has little published data.

In the vial

mg

Water added

mL

Your dose

mgmcg

Syringe

What do these boxes mean?

In the vial: The amount of powder in the vial before you add anything, e.g. “5 mg”. It’s on the label or the box.
Water added: How much bacteriostatic water you mixed in. It doesn’t change how much peptide you get, only how strong the mix is. More water makes each dose a bigger amount that’s easier to measure.
Your dose: How much peptide you want per injection, from your prescription or plan. Not the number of units on the syringe; that’s what we work out.
Syringe: Check the packet: 1 mL, 0.5 mL or 0.3 mL (sometimes written 100, 50 or 30 units). All are U-100: 100 units is 1 mL. Smaller syringes measure small doses more accurately.

When does this vial expire?

Mixed on

Good for (days)

NAD+ at a glance

Evidence: Limited human evidence. Sold as a supplement; injected use has little published data.
Vial sizes: 100, 250, 500, 1,000 mg
Usual dose range: 50–250 mg
How often: 2–3× weekly
Unmixed: Freezer −20°C
After mixing: Fridge 2–8°C, use within 28 days

NAD+ units to mg chart

Syringe units on any U-100 insulin syringe (100 units = 1 mL), worked out by the same engine as the calculator. Your own vial and water may differ: use the calculator for your numbers.

100 mg vial + 5 mL water (20 mg per mL)
Dose	Units	mL
50 mg	More than a 1 mL syringe
100 mg	More than a 1 mL syringe
200 mg	More than the vial holds
250 mg	More than the vial holds

250 mg vial + 5 mL water (50 mg per mL)
Dose	Units	mL
50 mg	100	1
100 mg	More than a 1 mL syringe
200 mg	More than a 1 mL syringe
250 mg	More than a 1 mL syringe

500 mg vial + 10 mL water (50 mg per mL)
Dose	Units	mL
50 mg	100	1
100 mg	More than a 1 mL syringe
200 mg	More than a 1 mL syringe
250 mg	More than a 1 mL syringe

1,000 mg vial + 10 mL water (100 mg per mL)
Dose	Units	mL
50 mg	50	0.5
100 mg	100	1
200 mg	More than a 1 mL syringe
250 mg	More than a 1 mL syringe

NAD+ questions

How many units is 50 mg of NAD+?

With a 100 mg vial mixed with 5 mL of bacteriostatic water, 50 mg is 250 units (2.5 mL) on a U-100 insulin syringe. A different vial size or amount of water changes this, so check it with your own numbers.

How much water do I add to a 100 mg NAD+ vial?

5 mL of bacteriostatic water is common, which makes 20 mg per mL. More water makes each dose a bigger amount that is easier to measure; it doesn't change how much is in the vial.

How long does mixed NAD+ last?

Once mixed, keep it at fridge temperature, 2–8°C and use it within 28 days. Unmixed powder: freezer −20°c.

What is NAD+?

NAD+ is a coenzyme, not a peptide: every cell uses it to make energy and repair DNA, and levels fall with age. It’s sold as injections, drips, nasal sprays and oral precursors (NMN and NR).

What the research shows

Raising NAD+ is well studied in animals. In people, trials so far show that levels go up, but not clear health benefits.

Big numbers

NAD+ doses are tens to hundreds of milligrams, so vials are large (100 to 1,000 mg) and take 5 to 10 mL of water. Injections often sting; injecting slowly is the usual advice.